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Murugan, M.
- Characterisation of native Bacillus thuringiensis isolates toxicity to fall armyworm, Spodoptera frugiperda (J.E. Smith)
Abstract Views :181 |
PDF Views:115
Authors
M. Maheesha
1,
V. Balasubramani
2,
M. Murugan
1,
M. Raveendran
3,
G. Rajadurai
3,
T. Tamilnayagan
3,
E. Kokiladevi
3,
N. Sathiah
1
Affiliations
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
2 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003,, IN
3 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
2 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003,, IN
3 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 35, No 3 (2021), Pagination: 171-180Abstract
Toxicity of nine indigenous Bacillus thuringiensis (Bt) isolates collected from Tamil Nadu, India were tested against fall armyworm, Spodoptera frugiperda. At 30 ?g/ml concentration, two Bt isolates viz., T350 and T532 recorded 100 per cent mortality whereas isolates T527 and T532 registered 96 per cent mortality against neonate larvae of S. frugiperda in leaf dip bioassay. SDS PAGE analysis of spore crystal mixture revealed the presence of Cry1 and Cry2 proteins with visible bands at 130 kDa and 65 kDa. PCR screening results showed the presence of cry1 (cry1A, cry1Aa, cry1Ab), cry2 (cry2Aa, cry2Ab) in four isolates and vip3A genes in three isolates but cry9 gene was not present in any of the isolates tested.
Keywords
Bacillus thuringiensis, bioassay, Cry Genes, fall armyworm, PCR, SDS PAGEFull Text
- Evaluation of Bio-Agents, Synthetic Insecticides and Organic Amendment Against the Root-Knot Nematode, Meloidogyne Spp. in Cardamom [Elettaria Cardamomum
Abstract Views :172 |
PDF Views:86
Authors
T. SATHYAN
1,
M. K. DHANYA
1,
M. MURUGAN
1,
K. ASHOKKUMAR
1,
T. S. ASWATHY
1,
R. NARAYANA
2,
K.B. DEEPTHY
3
Affiliations
1 Cardamom Research Station, Pampadumpara, KAU, Idukki – 685553, Kerala, IN
2 Department of Nematology, CoA, Vellayani, Thiruvanathapuram – 695522, Kerala, IN
3 Department of Agrl. Entomology, CoA, Vellanikkara, Thrissur – 680656, Kerala, IN
1 Cardamom Research Station, Pampadumpara, KAU, Idukki – 685553, Kerala, IN
2 Department of Nematology, CoA, Vellayani, Thiruvanathapuram – 695522, Kerala, IN
3 Department of Agrl. Entomology, CoA, Vellanikkara, Thrissur – 680656, Kerala, IN
Source
Journal of Biological Control, Vol 35, No 2 (2021), Pagination: 68-75Abstract
Investigations were made to study the effects of three nemato-pathogenic fungi viz. Purpureocillium lilacinum, P. lilacinum + neem cake, Pochonia chlamydosporia and Trichoderma sp. as well as two synthetic insecticides (cartap hydrochloride and carbosulfan) against the Root-knot Nematodes (RKN) of cardamom at the farmers’ fields in three locations of Idukki district, Kerala, India during 2016- 2019. Results revealed that the combined application of P. lilacinum with neem cake gave maximum reduction of typical symptoms of infestation like the leaf narrowing (6.42%) and root knot formation (1.32 nos.) followed by P. chlamydosporia with 8.04% narrowed leaves and 1.83 root knots. The application of P. lilacinum and neem cake in combination (0.82 kg/plant and 2.99) followed by P. chlamydosporia (0.71 kg/plant and 2.71 recorded highest yield and Benefit Cost Ratio, respectively). Bio-control agents are therefore efficient and environmentally safer for managing the RKN in cardamom. Future studies can be directed to focus on the compatibility and efficiency enhancement of these bio-agents with organic amendments.Keywords
Bio-agents, cardamom, Root Knot Nematode, insecticides and organic amendments, management.Full Text
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